Egyptian prosecutors referred Muslim Brotherhood Supreme Guide Mohamed Badie and other group leaders to trial for holding an "armed encampment" in Cairo's Rabaa Sqaure, a prosecution statement said.
The statement, which Aswat Masriya obtained a copy of, did not provide further details on the numbers or names of the other leaders who were referred to the criminal court.
Rabaa Square once hosted an encampment organised in late June 2013 to show solidarity with and support for ousted president Mohamed Mursi and his administration.
The encampment was set up days before Mursi's ouster on July 3, 2013 and was maintained for weeks after his ouster in July 2013 following mass protests against his rule.
Prosecutors said Badie and the other defendants organised the encampment and organised marches that targeted citizens in different locations. They are accused of staging an "armed gathering," arms possession and carrying out "terrorist acts," among various other charges.
In addition to the Rabaa encampment, a parallel but smaller encampment was set up for the same reason in Giza's Nahda square.
Both encampments were forcibly dispersed on August 14, 2013, leaving hundreds of protesters dead in what was described by Human Rights Watch as "the most serious incident of mass unlawful killings in modern Egyptian history."
The second anniversary to mark the dispersals coincides with next Friday.
The Rabaa square was renamed last month, to carry the name of assassinated prosecutor general Hisham Barakat.
The Muslim Brotherhood has faced a crackdown since mid-2013 and the group's leaders have mostly been placed behind bars.
Badie, the group's highest figure, has since received multiple death and life in prison sentences.
Egypt listed the Brotherhood as a terrorist organisation in December 2013.
The state insists the Brotherhood is behind the stringent wave of militancy which has targeted security personnel since mid-2013.
The Brotherhood continuously denies the accusations.
