Five Egyptian policemen were killed in a drive-by shooting on a checkpoint In Beni Suef governorate (200 km south of Cairo) on Thursday morning, Ahram Arabic news website reported.
Two policemen were also wounded in the attack.
"Masked gunmen on two motorcycles opened fire heavily on the forces at the checkpoint," the interiror ministry said in a statement.
Minor bomb attempts targeting state and security buildings have become commonplace following the army's ouster of Mohamed Morsi in July amid mass protests.
The authorities have carried out a sustained crackdown on Morsi's Brotherhood, which was recently designated a terrorist organisation.
A bomb exploded outside a Cairo court just before polls were to set to open in last week's constitution referendum, leaving no casualties.
A spate of violence in densely populated areas has raised fears that militant activity in the Sinai Peninsula, which has spiked since Morsi's removal, is spreading to other parts of the country.